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KMID : 0608520120180010075
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Korean Journal of Oriental Medicine
2012 Volume.18 No. 1 p.75 ~ p.84
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Effects of Sophorae Radix on Human Breast Adenocarcinoma Cells
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Lee Hee-Jung
Kim Min-Chul
Lim Bo-Ra
Bae Go-Eun
Kim Hyung-Woo
Kwon Young-Kyu
Kim Byung-Joo
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Abstract
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Objective: The purpose of this study was to investigate the anti-cancer effects of Sophorae Radix and the effects of Doxorubicin (DOX) in human breast adenocarcinoma cells (MCF-7).
Method: We used human breast adenocarcinoma cell line, MCF-7 cells. We examined cell death by MTT assay and caspase 3 assay with Sophorae Radix. To examine the inhibitory effects of Sophorae Radix, cell cycle analysis was done the MCF-7 cells after three days with Sophorae Radix. The reversibility of Sophorae Radix was examined on one day to five days treatment with 100 ㎍/ml Sophorae Radix.
Result: Sophorae Radix inhibited the growth of MCF-7 cells in a dose-dependent fashion. Also we showed that Sophorae Radix induced apoptosis in MCF-7 cells by MTT assay, caspase 3 assay and sub-G1 analysis. Sophorae Radix combined with DOX markedly inhibited the growth of MCF-7 cells compared to Sophorae Radix or DOX alone. After 3 days treatment of MCF-7 cells with Sophorae Radix, the fraction of cells in sub-G1 phase was much higher than that of the control group.
Conclusion: Our findings provide insight into unraveling the effects of Sophorae Radix in human breast adenocarcinoma cells and developing therapeutic agents against breast cancer.
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KEYWORD
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Sophorae Radix, Human Breast Adenocarcinoma Cell, MCF-7, Doxorubicin (DOX)
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